This thesis researches the transatlantic relation between the US and the EU regarding counterterrorism. Both their individual counterterrorism strategies and their joint declarations were analyzed, against the background of the theories of strategic culture and multilaterism vs. unilaterism. Although both actors have been working closely together, there are some differences between their strategic cultures. By using the theoretical concept of strategic culture, I endeavor to distinguish the reasons why the US tends to use force in order to protect homeland security and the EU more believes in diplomatic means. The political establishment in the US has challenged the transatlantic relationship. On the other hand, the European complex governmental system has also influenced the cooperation. The factors used to determine if the transatlantic relation has been undermined are: the US’ approach to individual member states, difference in threat perceptions, multilaterism vs. unilaterism and discourse. The conclusion is that the transatlantic cooperation was to a certain extent undermined, but has never been destroyed. The transatlantic relation regarding counterterrorism has been closer than before 9/11, and exists next to the individual strategies and measures, but is certainly a relevant contribution in fighting terrorism.